Ditra, cement board, Hardiebacker, and foam boards like GoBoard are all reasonable substrate options for floor tile. On the board products you generally only need the 1/4" thick version for floor applications, since they are continually supported by the plywood subfloor. [Note that if you had a dimensional lumber subfloor, a layer of plywood as intermediate between the solid sawn wood and the tile backer is generally called for, since dimensional lumber will move more with temperature/humidity changes.]
So the choice of whether to leave the underlayment layer of plywood is up to you, based on its condition and what height you want to net out at. I would probably only leave it if its face is in good condition and (a) it would be a pain to remove or (b) I wanted the extra height. Unless the underlayment plywood is nearly as thick as the subfloor plywood, the underlayment plywood doesn't provide significant structural benefit.
Whatever tile substrate you choose, be sure to read and follow the manufacturer's instructions.
Cheers, Wayne